Deciphering the function of YDL167c in Saccharomyces cerevisiae
Article Sidebar
Main Article Content
Abstract
Saccharomyces cerevisiae, a budding yeast, is a single-celled eukaryotic organism used to study molecular and cellular processes and pathways. Although the DNA sequence of all yeast genes is known, the function of over 1000 yeast genes is unknown. In this study, the potential function of YDL167c was examined using a mix of bioinformatics and wet lab experiments. Using the DNA and protein sequence of YDL167c, biological databases, such as SGD and LoQatE, were searched to find likely protein domains and cellular localization information. The results of these investigations showed this gene is found in the cytoplasmic stress granules and is most likely involved in the creation and stabilization of ribosomes. Next, a yeast strain bearing a knock-out of YDL167c was generated using a PCR-based method. Successful disruption of YDL167c with the URA3 gene was verified using a second PCR reaction. Finally, spot assays and morphological analyses were performed to compare the growth of ydl167cΔ yeast to the wild-type strain. Results of these experiments will be presented.
Downloads
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
- Student authors waive FERPA rights for only the publication of the author submitted works.
Specifically: Students of Indiana University East voluntarily agree to submit their own works to The Journal of Student Research at Indiana University East, with full understanding of FERPA rights and in recognition that for this one, specific instance they understand that The Journal of Student Research at Indiana University East is Public and Open Access. Additionally, the Journal is viewable via the Internet and searchable via Indiana University, Google, and Google-Scholar search engines.
References
References
Beltran JL, Bain RK, Stiefel MJ, McDonnell AS, Carr NN, and Thurtle-Schmidt BH. Human SLC4A11
does not complement BOR1 or support borate transport in Saccharomyces cerevisiae. MicroPubl Biol.
Jul 20;2022:10.17912/micropub.biology.000605. doi: 10.17912/micropub.biology.000605. PMID:
; PMCID: PMC9315404.
Lõoke , M., Kristjuhan, K., & Kristjuhan, A. (2011). Extraction of genomic DNA from yeasts for PCRbased
applications. Biotechniques, 50(5), 325-328. https://www.ncbi.nlm.nih.gov/pubmed/21548894
Gietz, RD, and Schiestl, RH (2007) Frozen competent yeast cells that can be transformed with high
efficiency using the LiAc/SS carrier DNA/PEGmethod. Nat Protoc 2: 1–4 nprot.2007.17.pdf
Nakamasu S, Kikuma T, Hashiguchi Y, Tada S, Sano K, Ito Y, Takeda Y. Phenotypic analysis of
α1,2-mannosidase-like protein deletion mutants in Saccharomyces cerevisiae. MicroPubl Biol.
Sep 23;2022:10.17912/micropub.biology.000640. doi: 10.17912/micropub.biology.000640. PMID:
; PMCID: PMC9547274.