Effects of yjr118c gene deletion on growth of S. cerevisiae
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Abstract
To determine effects of a gene deletion of YJR118C in the S. cerevisiae yeast strain and the function of this gene within yeast, several techniques were utilized. YJR118C, a part of the ILM1 family, was replaced with the URA3 gene to observe effects at the cellular level. Bioinformatics research and wet lab procedures were completed to observe this gene further. Gene deletion, two rounds of spot assays, confirmation PCR, and morphological analysis were all utilized to obtain results. Our results suggest that YJR118C is cytoplasmic with a chance of being localized to the endoplasmic reticulum of the cell, specifically relating to ER retention signals.
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References
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