Ergosterol Transportation and YHR039C in Saccharomyces cerevisiae
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Abstract
The budding yeast Saccharomyces cerevisiae is a great model organism because it is a single-celled eukaryote, in which the genome has been sequenced entirely. Though the whole genome has been sequenced there are still genes that have unknown functions. The goal of this study is to find the potential function of the ORFan, YHR039C. Through a series of experiments the function of YHR039C was examined using bioinformatic searches and wet lab experiments. Using YHR039C’s DNA and protein sequences, many databases were used to find information like protein domains and cellular localization. The results from the bioinformatic investigations showed YHR039C is in the endoplasmic reticulum and affects structure. Then using a series of PCR reactions, the strain that had the knock-out of YHR039C was created, and then the disruption of YHR039C was confirmed using another PCR reaction. Lastly through multiple spot assays and morphological analyses, the growth of yhr039C yeast was compared to the wild-type strain.
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References
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