README FILE FOR Datasets for Identification and characterization of human proteoforms by top-down LC-21 tesla FT-ICR mass spectrometry [PERMANENT URL GOES HERE] Created by: Caroline DeHart Proteomics Center of Excellence Northwestern University 2170 Campus Drive Silverman Hall 3710 Evanston, IL 60208 caroline.dehart@northwestern.edu --------------------------------------- FILE LIST ./Metadata.txt ./README.txt ./DLD-1_A/LCA_CD_DLD1par_10per_f1_freshdil3x_LCA_IPA_gradient_v9_300A_5um_PoroshellC8_01_alt_param.raw ./DLD-1_A/LCA_CD_DLD1par_10per_f2_freshdil3x_LCA_IPA_gradient_v9_300A_5um_PoroshellC8_02_alt_param.raw ./DLD-1_A/LCA_CD_DLD1par_10per_f3_freshdil3x_LCA_IPA_gradient_v9_300A_5um_PoroshellC8_03_alt_param_REDO.raw ./DLD-1_A/LCA_CD_DLD1par_10per_f4_freshdil3x_LCA_IPA_gradient_v9_300A_5um_PoroshellC8_04_alt_param.raw ./DLD-1_A/LCA_CD_DLD1par_10per_f5_freshdil3x_LCA_IPA_gradient_v9_300A_5um_PoroshellC8_05_alt_param.raw ./DLD-1_A/LCA_CD_DLD1par_10per_f6_freshdil3x_LCA_IPA_gradient_v9_300A_5um_PoroshellC8_06_alt_param.raw ./DLD-1_A/LCA_CD_DLD1par_10per_f7_freshdil3x_LCA_IPA_gradient_v9_300A_5um_PoroshellC8_07_alt_param.raw ./DLD-1_A/LCA_CD_DLD1par_10per_f8_freshdil3x_LCA_IPA_gradient_v9_300A_5um_PoroshellC8_08_alt_param.raw ./DLD-1_B/LCA_CD_02062016_DLD1_parA1_10per_f1_50K_CID_90min.raw ./DLD-1_B/LCA_CD_02062016_DLD1_parA1_10per_f1_50K_ETD_90min.raw ./DLD-1_B/LCA_CD_02062016_DLD1_parA1_10per_f2_50K_CID_90min.raw ./DLD-1_B/LCA_CD_02062016_DLD1_parA1_10per_f2_50K_ETD_90min.raw ./DLD-1_B/LCA_CD_02062016_DLD1_parA1_10per_f3_50K_CID_90min.raw ./DLD-1_B/LCA_CD_02062016_DLD1_parA1_10per_f3_50K_ETD_90min.raw ./DLD-1_B/LCA_CD_02062016_DLD1_parA1_10per_f4_50K_CID_90min.raw ./DLD-1_B/LCA_CD_02062016_DLD1_parA1_10per_f4_50K_ETD_90min.raw ./DLD-1_B/LCA_CD_02052016_DLD1_parA1_10per_f5_100K_CID_120min_025q.raw ./DLD-1_B/LCA_CD_02052016_DLD1_parA1_10per_f5_100K_ETD_120min_12ms.raw ./DLD-1_B/LCA_CD_02052016_DLD1_parA1_10per_f6_100K_CID_120min_025q.raw ./DLD-1_B/LCA_CD_02052016_DLD1_parA1_10per_f6_100K_ETD_120min_12ms.raw ./DLD-1_B/LCA_CD_02052016_DLD1_parA1_10per_f7_100K_CID_120min_025q.raw ./DLD-1_B/LCA_CD_02052016_DLD1_parA1_10per_f7_100K_ETD_120min_12ms.raw ./DLD-1_B/LCA_CD_02052016_DLD1_parA1_10per_f8_100K_CID_120min_025q.raw ./DLD-1_B/LCA_CD_02052016_DLD1_parA1_10per_f8_100K_ETD_120min_12ms.raw ./DLD-1_C/LCA_CD_01312016_DLD1_parC2_10per_f1_50K_CID_90min.raw ./DLD-1_C/LCA_CD_01312016_DLD1_parC2_10per_f1_50K_ETD_90min.raw ./DLD-1_C/LCA_CD_01312016_DLD1_parC2_10per_f2_50K_CID_90min.raw ./DLD-1_C/LCA_CD_01312016_DLD1_parC2_10per_f2_50K_ETD_90min.raw ./DLD-1_C/LCA_CD_01312016_DLD1_parC2_10per_f3_50K_CID_90min.raw ./DLD-1_C/LCA_CD_01312016_DLD1_parC2_10per_f3_50K_ETD_90min.raw ./DLD-1_C/LCA_CD_01312016_DLD1_parC2_10per_f4_50K_CID_90min.raw ./DLD-1_C/LCA_CD_01312016_DLD1_parC2_10per_f4_50K_ETD_90min.raw ./DLD-1_C/LCA_CD_02032016_DLD1_parC2_10per_f5_100K_CID_120min_025q.raw ./DLD-1_C/LCA_CD_02032016_DLD1_parC2_10per_f5_100K_ETD_120min_12ms.raw ./DLD-1_C/LCA_CD_02032016_DLD1_parC2_10per_f6_100K_CID_120min_025q.raw ./DLD-1_C/LCA_CD_02032016_DLD1_parC2_10per_f6_100K_ETD_120min_12ms.raw ./DLD-1_C/LCA_CD_02032016_DLD1_parC2_10per_f7_100K_CID_120min_025q.raw ./DLD-1_C/LCA_CD_02032016_DLD1_parC2_10per_f7_100K_ETD_120min_12ms.raw ./DLD-1_C/LCA_CD_02032016_DLD1_parC2_10per_f8_100K_CID_120min_025q.raw ./DLD-1_C/LCA_CD_02032016_DLD1_parC2_10per_f8_100K_ETD_120min_12ms.raw ./21T_DLD-1_A.tdReport ./21T_DLD-1_B.tdReport ./21T_DLD-1_C.tdReport ./Homo_Sapiens_2016_04.txt --------------------------------------- FILE INFORMATION DLD-1 A, DLD-1 B, and DLD-1 C All samples were generated by separation of human whole-cell lysate by GELFrEE (10%T), followed by single (A) or duplicate (B and C) injections of fractions 1-8 for analysis by top2 data-dependent FT-MS/MS on a 21 T FT-ICR mass spectrometer, using either CID or fETD fragmentation. The resulting .raw files were preprocessed and searched against the SwissProt_Homo_Sapiens_April_2016 database using a customized version of TDPortal. --------------------------------------- RESEARCH QUESTION(S) We demonstrate high-throughput top-down proteomic analysis of human colorectal cancer cell lysate by LC-MS/MS on a 21 T FT-ICR mass spectrometer. --------------------------------------- CHANGELOG 0. Uploading of files --------------------------------------- COPYRIGHT & LICENSING INFORMATION This data is licensed for reuse under a Creative Commons Attribution 4.0 license. --------------------------------------- LIMITATIONS No limitations are present in the current files.