Generating Biosensors with Fluorescent Proteins in E. coli
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The ASURE BIOT-X150 laboratory course works through the process of generating a better redox fluorescent biosensor in Escherichia coli. Biosensors are devices designed to detect specific chemical signals and produce a measurable response. Fluorescent biosensors placed in the periplasm of E. coli can detect redox reactions by emitting fluorescence. The redox environment of the periplasm is linked to bacterial pathogenesis, so these biosensors could aid in the discovery of antibiotics. However, previous biosensors have faced limitations restricting their effectiveness. Our objective is to develop a biosensor that overcomes these limitations. We will incorporate the fluorescent protein mEYFP into a biosensor and introduce it into the periplasm of E. coli. The fluorescence output of each plasmid will then be measured and analyzed to assess the efficiency of the newly engineered biosensor. Hopefully, we will be able to achieve the goals of the objective.