Identification and characterization of human proteoforms by top-down LC-21 tesla FT-ICR mass spectrometry

Simple item page
dc.altmetrics.displaytrue
dc.contributor.authorAnderson, Lissa C
dc.contributor.authorDeHart, Caroline J
dc.contributor.authorKaiser, Nathan K
dc.contributor.authorFellers, Ryan T
dc.contributor.authorSmith, Donald F
dc.contributor.authorGreer, Joseph B
dc.contributor.authorLeDuc, Richard D
dc.contributor.authorBlackney, Gregory T
dc.contributor.authorThomas, Paul M
dc.contributor.authorKelleher, Neil L
dc.contributor.authorHendrickson, Christopher L
dc.date.accessioned2016-07-18T18:03:43Z
dc.date.available2016-07-18T18:03:43Z
dc.date.issued2016
dc.descriptionCollection of RAW files, .tdReport files, and search database information for the abovementioned research article.
dc.description.abstractThe National High Magnetic Field Laboratory (NHMFL) recently installed a 21T FT-ICR mass spectrometer, which is part of the NHMFL FT-ICR User Facility and available to all qualified users. Here, we demonstrate top-down LC-21 T FT-ICR MS/MS of intact proteins derived from human colorectal cancer cell lysate. We identify a combined total of 684 unique protein entries observed as 3,238 unique proteoforms at a 1% false discovery rate, based on rapid, data-dependent acquisition of collision-induced and electron-transfer dissociation tandem mass spectra from 40 LC-MS/MS experiments.
dc.description.sponsorshipThis work was supported by the National Science Foundation Cooperative Agreement No. DMR-1157490 and the State of Florida as well as by the National Institute of General Medical Science (P41GM108569) for the National Resource for Translational and Developmental Proteomics (NRTDP) based at Northwestern University.
dc.identifier.citationForthcoming
dc.identifier.doiForthcoming
dc.identifier.urihttps://hdl.handle.net/2022/20924
dc.language.isoen_US
dc.publisherAmerican Chemical Society
dc.relation.ispartofseriesForthcoming;Forthcoming
dc.relation.isversionofForthcoming
dc.rightsCreative Commons Attribution 4.0 license (http://creativecommons.org/licenses/by/4.0/)
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectFT-ICR, 21 tesla, Top-down proteomics, ultra-high resolution, FTMS
dc.titleIdentification and characterization of human proteoforms by top-down LC-21 tesla FT-ICR mass spectrometry
dc.typeDataset

Files

Original bundle

Now showing 1 - 1 of 1
No Thumbnail Available
Name:
README_Anderson_DeHart_21T.txt
Size:
4.56 KB
Format:
Plain Text
Download

Collections

Data
Can’t use the file because of accessibility barriers? Contact us