Microsatellite PCR and ABI3730 Genotyping Instructions

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Molecular_Microsatellite PCR and ABI3730 Genotyping instructions.pdf (265.53 KB)
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2013-03-29

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https://hdl.handle.net/2022/15403

Abstract

Protocol for PCR of extracted avian DNA. Includes directions for PCR to amplify microsatellite loci (used for paternity analysis) and for PCR to amplify sex-specific loci (used for determining sample sex). Includes directions for PCR reactions as well as analysis of PCR product on the ABI3730. Also includes information on the primers used by the Ketterson lab for analyzing junco microsatellite loci.

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protocol, DNA, PCR, birds, sex, genotyping, microsatellites, lab

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 http://creativecommons.org/licenses/by-nc-sa/3.0/us/

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Molecular Protocols

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Lab Protocols