Effects of p53R248W on Proliferation and Chemoresistance of Human Lung Cancer

Abstract

As a commonly mutated gene in human cancers, TP53 is a key target for cancer therapies. A single mutation can have variable cancer-promoting effects depending on other mutations present within the tumor. Currently available cell systems fail to accurately represent the complexity and heterogeneity of mutations present in clinically isolated tumors. In the present study, liposome-mediated transfection of mutant p53R248W into p53-null H1299 human lung cancer cells was used to manufacture a system that more accurately represents patient tumors. This system can be used to better understand the contribution of this mutation to lung tumor progression and resistance to treatment.