De novo transcriptome sequencing in a songbird, the dark-eyed junco (Junco hyemalis): Genomic tools for an ecological model system
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Date
2012-07-09
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BMC Genomics
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Abstract
Though genomic-level data are becoming widely available, many of the metazoan species sequenced are laboratory systems whose natural history is not well documented. In contrast, the wide array of species with very well-characterized natural history have, until recently, lacked genomics tools. It is now possible to address significant evolutionary genomics questions by applying high-throughput sequencing to discover the majority of genes for ecologically tractable species, and by subsequently developing microarray platforms from which to investigate gene regulatory networks that function in natural systems. We used GS-FLX Titanium Sequencing (Roche/454-Sequencing) of two normalized libraries of pooled RNA samples to characterize a transcriptome of the dark-eyed junco (Junco hyemalis), a North American sparrow that is a classically studied species in the fields of photoperiodism, speciation, and hormone-mediated behavior.
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Keywords
Transcriptome, Aves, pyrosequencing, microarray, Junco, 454 titanium cDNA sequencing, single nucleotide polymorphism
Citation
Peterson et al. : De novo transcriptome sequencing in a songbird, the dark-eyed junco ( Junco hyemalis ): genomic tools for an ecological model system. BMC Genomics 2012 13 :305.
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http://creativecommons.org/licenses/by-nc-sa/3.0/us/
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
By downloading this document or using any information contained therein, you agree to the Creative Commons Attribution-NonCommercial-ShareAlike (CC BY-NC-SA) license terms, which explain terms governing use, creation of derivative research, and requirements for citing the document.
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
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Article