Fluorescent Proteins As A More Quantifiable Approach Than Chromogenic Redox Biosensors

Abstract

A biosensor is a tool/device that has the ability to detect for a specific biological or chemical substance. Fluorescent biosensors are harnessed in relation to Escherichia coli bacteria so as to quantify the presence of redox reactions via the expression of fluorescence. Because the redox environment is linked to bacterial pathogenesis, this process will lead to the discovery of novel antibiotics combating bacteria. Existing biosensors in this area of study are associated with limitations that hinder their adaptability when applied to a wide variety of situations. So, the goal moving forward is to generate a biosensor that can overcome this obstacle. In order to execute this ideal, the fluorescent protein, mKate, will be developed as a biosensor and guided toward the periplasm of two variants of E. coli. Fluorescent outputs will be analyzed and employed to improve the newly constructed biosensor through directed evolution.