Protocol for steroid radioimmunoassay of egg yolks

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Radioimmunoassay_Egg yolk RIA Protocol.pdf (267.65 KB)
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Date

2013-03-29

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https://hdl.handle.net/2022/15417

Abstract

This protocol can be used to measure the concentration of steroid hormones in yolk samples. This protocol has been copied and modified from protocols provided by Dr. John Wingfield at the University of Washington and Dr. Hubert Schwabl at Washington State University. It has been written for use in the laboratory of Dr. Ellen Ketterson at Indiana University, and therefore the details may require modification if the procedure is performed elsewhere. The general procedure, which takes 5 days to complete, is described below. Variations and additional information are provided in the appendices. Prepared October 1997 by Joe Lipar.

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hormones, endocrinology, corticosterone, cort, assay, lab, eggs, egg yolk, testosterone, progesterone, RIA

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By downloading this document or using any information contained therein, you agree to the Creative Commons Attribution-NonCommercial-ShareAlike (CC BY-NC-SA) license terms, which explain terms governing use, creation of derivative research, and requirements for citing the document.
 http://creativecommons.org/licenses/by-nc-sa/3.0/us/

Type

Radioimmunoassay Protocol

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Lab Protocols