Show simple item record Rizk, Shahir S. Kouadio, Jean-Louis K. Szymborska, Anna Duguid, Erica M. Mukherjee, Somnath S. Zheng, Jiamao Clevenger, Charles V. Kossiakoff, Anthony A. 2021-06-17T19:12:15Z 2021-06-17T19:12:15Z 2015
dc.identifier.citation Rizk, Shahir S., et al. “Engineering Synthetic Antibody Binders for Allosteric Inhibition of Prolactin Receptor Signaling.” Cell Communication and Signaling, vol. 13, no. 1, Jan. 2015
dc.description.abstract Background: Many receptors function by binding to multiple ligands, each eliciting a distinct biological output. The extracellular domain of the human prolactin receptor (hPRL-R) uses an identical epitope to bind to both prolactin (hPRL) and growth hormone (hGH), yet little is known about how each hormone binding event triggers the appropriate response. Findings: Here, we utilized a phage display library to generate synthetic antibodies (sABs) that preferentially modulate hPRL-R function in a hormone-dependent fashion. We determined the crystal structure of a sAB-hPRL-R complex, which revealed a novel allosteric mechanism of antagonism, whereby the sAB traps the receptor in a conformation more suitable for hGH binding than hPRL. This was validated by examining the effect of the sABs on hormone internalization via the hPRL-R and its downstream signaling pathway. Conclusions: The findings suggest that subtle structural changes in the extracellular domain of hPRL-R induced by each hormone determine the biological output triggered by hormone binding. We conclude that sABs generated by phage display selection can detect these subtle structural differences, and therefore can be used to dissect the structural basis of receptor-ligand specificity. Keywords: Prolactin signaling, Synthetic antibody, Phage display, Allostery en
dc.format.extent 6 pages
dc.format.mimetype PDF
dc.language.iso en en
dc.publisher BMC en
dc.subject.lcsh Immunoglobulins
dc.subject.lcsh Synthetic biology
dc.subject.lcsh Prolactin
dc.title Engineering synthetic antibody binders for allosteric inhibition of prolactin receptor signaling en
dc.type Article en

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