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dc.contributor.author Capshew, C.R. en
dc.contributor.author Dusenbury, K.L. en
dc.contributor.author Hundley, H.A. en
dc.date.accessioned 2014-11-11T21:06:20Z en
dc.date.available 2014-11-11T21:06:20Z en
dc.date.issued 2012 en
dc.identifier.citation Capshew, C. R., Dusenbury, K. L., & Hundley, H. A. (2012). Inverted Alu dsRNA structures do not affect localization but can alter translation efficiency of human mRNAs independent of RNA editing. Nucleic Acids Research, 40(17), 8637-8645. http://dx.doi.org/10.1093/nar/gks590 en
dc.identifier.uri http://hdl.handle.net/2022/19125
dc.description.abstract With over one million copies, Alu elements are the most abundant repetitive elements in the human genome. When transcribed, interaction between two Alus that are in opposite orientation gives rise to double-stranded RNA (dsRNA). Although the presence of dsRNA in the cell was previously thought to only occur during viral infection, it is now known that cells express many endogenous small dsRNAs, such as short interfering RNA (siRNAs) and microRNA (miRNAs), which regulate gene expression. It is possible that long dsRNA structures formed from Alu elements influence gene expression. Here, we report that human mRNAs containing inverted Alu elements are present in the mammalian cytoplasm. The presence of these long intramolecular dsRNA structures within 3′-UTRs decreases translational efficiency, and although the structures undergo extensive editing in vivo, the effects on translation are independent of the presence of inosine. As inverted Alus are predicted to reside in >5 of human protein-coding genes, these intramolecular dsRNA structures are important regulators of gene expression. en
dc.language.iso en_US en
dc.publisher Oxford University Press en
dc.relation.isversionof https://doi.org/10.1093/nar/gks590 en
dc.rights © 2012 The Authors. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. en
dc.rights.uri http://creativecommons.org/licenses/by-nc/3.0 en
dc.subject double stranded RNA en
dc.subject inosine en
dc.subject messenger RNA en
dc.subject 3' untranslated region en
dc.subject Alu sequence en
dc.subject cell strain HEK293 en
dc.subject controlled study en
dc.subject cytoplasm en
dc.subject Gene Expression Regulation en
dc.subject HeLa cell en
dc.subject human en
dc.subject human cell en
dc.subject Lentivirinae en
dc.subject Lentivirus infection en
dc.subject nonhuman en
dc.subject reverse transcription polymerase chain reaction en
dc.subject RNA editing en
dc.subject RNA isolation en
dc.subject RNA structure en
dc.subject RNA translation en
dc.subject Cytoplasm en
dc.subject Gene Expression Regulation en
dc.subject HEK293 Cells en
dc.subject Protein Biosynthesis en
dc.subject RNA, Double-Stranded en
dc.subject RNA, Messenger HEK293 Cells en
dc.subject inosine, 58-63-9 en
dc.title Inverted Alu dsRNA structures do not affect localization but can alter translation efficiency of human mRNAs independent of RNA editing en
dc.type Article en
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