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dc.contributor.author Liu, J. en
dc.contributor.author Mconnell, K. en
dc.contributor.author Dixon, M. en
dc.contributor.author Calvi, B.R. en
dc.date.accessioned 2014-11-10T18:09:56Z en
dc.date.available 2014-11-10T18:09:56Z en
dc.date.issued 2012 en
dc.identifier.citation Liu, J., McConnell, K., Dixon, M., & Calvi, B. R. (2012). Analysis of model replication origins in drosophila reveals new aspects of the chromatin landscape and its relationship to origin activity and the prereplicative complex. Molecular Biology of the Cell, 23(1), 200-212. http://dx.doi.org/10.1091/mbc.E11-05-0409 en
dc.identifier.uri http://hdl.handle.net/2022/19122
dc.description.abstract Epigenetic regulation exerts a major influence on origins of DNA replication during development. The mechanisms for this regulation, however, are poorly defined. We showed previously that acetylation of nucleosomes regulates the origins that mediate developmental gene amplification during Drosophila oogenesis. Here we show that developmental activation of these origins is associated with acetylation of multiple histone lysines. Although these modifications are not unique to origin loci, we find that the level of acetylation is higher at the active origins and quantitatively correlated with the number of times these origins initiate replication. All of these acetylation marks were developmentally dynamic, rapidly increasing with origin activation and rapidly declining when the origins shut off and neighboring promoters turn on. Fine-scale analysis of the origins revealed that both hyperacetylation of nucleosomes and binding of the origin recognition complex (ORC) occur in a broad domain and that acetylation is highest on nucleosomes adjacent to one side of the major site of replication initiation. It was surprising to find that acetylation of some lysines depends on binding of ORC to the origin, suggesting that multiple histone acetyltransferases may be recruited during origin licensing. Our results reveal new insights into the origin epigenetic landscape and lead us to propose a chromatin switch model to explain the coordination of origin and promoter activity during development. en
dc.language.iso en_US en
dc.publisher The American Society for Cell Biology en
dc.relation.isversionof https://doi.org/10.1091/mbc.E11-05-0409 en
dc.rights © 2012 Liu et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). en
dc.rights.uri http://creativecommons.org/licenses/by-nc-sa/3.0 en
dc.subject DNA en
dc.subject histone en
dc.subject histone acetyltransferase en
dc.subject origin recognition complex en
dc.subject acetylation en
dc.subject chromatin structure en
dc.subject controlled study en
dc.subject correlational study en
dc.subject DNA binding en
dc.subject DNA modification en
dc.subject DNA replication en
dc.subject Drosophila en
dc.subject female en
dc.subject gene amplification en
dc.subject gene locus en
dc.subject genetic regulation en
dc.subject male en
dc.subject molecular dynamics en
dc.subject nonhuman en
dc.subject nucleosome en
dc.subject oocyte development en
dc.subject priority journal en
dc.subject Animals en
dc.subject DNA Replication Timing en
dc.subject Drosophila Proteins en
dc.subject Epigenesis, Genetic en
dc.subject Gene Expression Regulation, Developmental en
dc.subject Models, Genetic en
dc.subject Multiprotein Complexes en
dc.subject Nucleosomes en
dc.subject Replication Origin en
dc.subject DNA, 9007-49-2 en
dc.subject histone, 9062-68-4 en
dc.subject histone acetyltransferase, 9054-51-7 en
dc.subject Chromatin en
dc.subject Histone Acetyltransferases, 2.3.1.48 en
dc.title Analysis of model replication origins in Drosophila reveals new aspects of the chromatin landscape and its relationship to origin activity and the prereplicative complex en
dc.type Article en
dc.altmetrics.display false en


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