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De novo transcriptome sequencing in a songbird, the dark-eyed junco (Junco hyemalis): Genomic tools for an ecological model system

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dc.contributor.author Peterson, Mark P.
dc.contributor.author Whittaker, Danielle J.
dc.contributor.author Ambreth, Shruthi
dc.contributor.author Sureschandra, Suhas
dc.contributor.author Buechlein, Aaron
dc.contributor.author Podicheti, Ram
dc.contributor.author Choi, Jeong-Hyeon
dc.contributor.author Lai, Zhao
dc.contributor.author Mockatis, Keithanne
dc.contributor.author Colbourne, John
dc.contributor.author Tang, Haixu
dc.contributor.author Ketterson, Ellen D.
dc.date.accessioned 2013-10-01T15:59:16Z
dc.date.available 2013-10-01T15:59:16Z
dc.date.issued 2012-07-09
dc.identifier.citation Peterson et al. : De novo transcriptome sequencing in a songbird, the dark-eyed junco ( Junco hyemalis ): genomic tools for an ecological model system. BMC Genomics 2012 13 :305. en_US
dc.identifier.uri http://www.biomedcentral.com/1471-2164/13/305 en_US
dc.identifier.uri http://hdl.handle.net/2022/16820
dc.description.abstract Though genomic-level data are becoming widely available, many of the metazoan species sequenced are laboratory systems whose natural history is not well documented. In contrast, the wide array of species with very well-characterized natural history have, until recently, lacked genomics tools. It is now possible to address significant evolutionary genomics questions by applying high-throughput sequencing to discover the majority of genes for ecologically tractable species, and by subsequently developing microarray platforms from which to investigate gene regulatory networks that function in natural systems. We used GS-FLX Titanium Sequencing (Roche/454-Sequencing) of two normalized libraries of pooled RNA samples to characterize a transcriptome of the dark-eyed junco (Junco hyemalis), a North American sparrow that is a classically studied species in the fields of photoperiodism, speciation, and hormone-mediated behavior. en_US
dc.language.iso en_US en_US
dc.publisher BMC Genomics en_US
dc.rights CC-BY-NC-SA
dc.rights By downloading this document or using any information contained therein, you agree to the Creative Commons Attribution-NonCommercial-ShareAlike (CC BY-NC-SA) license terms, which explain terms governing use, creation of derivative research, and requirements for citing the document.
dc.rights.uri http://creativecommons.org/licenses/by-nc-sa/3.0/us/
dc.rights.uri http://creativecommons.org/licenses/by-nc-sa/3.0/us/
dc.subject Transcriptome, Aves, pyrosequencing, microarray, Junco, 454 titanium cDNA sequencing, single nucleotide polymorphism en_US
dc.title De novo transcriptome sequencing in a songbird, the dark-eyed junco (Junco hyemalis): Genomic tools for an ecological model system en_US
dc.type Article en_US
dc.identifier.doi 10.1186/1471-2164-13-305


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