Protocol for steroid radioimmunoassay of plasma

Abstract
This protocol can be used to measure the concentration of steroid hormones in small plasma samples. This protocol has been copied and modified from protocols provided by Dr. John Wingfield at the University of Washington. It has been written for use in the laboratory of Dr. Ellen Ketterson at Indiana University, and therefore the details may require modification if the procedure is performed elsewhere. The general procedure, which takes 4-5 days to complete, is described below. Prepared January 1998 by Joe Casto, modified December 2005 by Jodie Jawor and May 2010 by Danielle Whittaker.
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Keywords
hormones, endocrinology, corticosterone, cort, assay, lab, plasma, testosterone, progesterone, RIA
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Type
Radioimmunoassay Protocol
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