Short protocol for direct radioimmunoassay of corticosterone (a.k.a. Little B)

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2013-03-29
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This protocol can be used to measure the concentration of corticosterone in small plasma samples. This protocol has been copied and modified from protocols provided by Dr. John Wingfield at the University of Washington. It has been written for use in the laboratory of Dr. Ellen Ketterson at Indiana University, and therefore the details may require modification if the procedure is performed elsewhere. The general procedure, which takes 4 days to complete, is described below. Variations and additional information are provided in the appendices. Prepared October 1999 by Joe Casto, modified October 2003 by Jodie Jawor.
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hormones, endocrinology, corticosterone, cort, assay, lab, plasma, little b, RIA
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Radioimmunoassay Protocol
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