Browsing Lab Protocols by Title

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  • Christine Bergeon Burns, Kimberly Rosvall (2013-03-29)
    Protocol for reverse transcriptase / cDNA synthesis from extracted RNA samples.
  • Joe Casto, Lynn Siefferman, Christine Bergeon Burns (2013-03-29)
    Recipe for mixing a solution of gonadotropin releasing hormone (c-GnRH) from a powder form.
  • Kimberly Rosvall, Dustin Reichard (2013-03-29)
    Protocol for corticosterone EIA, based on the CORT kit from Caymen
  • Jen Grindstaff (2013-03-29)
    Protocol to prepare frozen egg yolks for use in a variety of enzyme-linked immunosorbent assays (ELISAs).
  • Unsure original creator, possibly updated by Kristal Cain? (2013-03-29)
    Protocol to prepare frozen egg yolks for use in a variety of laboratory tests (ELIZA, Hormone assay, Carotenoid HPLC)
  • Steve Schoech (2013-03-29)
    A spreadsheet for calculating steroid hormone concentrations from radioimmunoassay results. Calculates concentrations of progesterone, testosterone, estradiol, corticosterone, and dihydrotestosterone.
  • Joe Casto, Joel McGlothlin, Ellen Ketterson (2013-03-29)
    Protocol for administering a GnRH challenge to a songbird. This procedure injects a bird with a known concentration of gonadotropin releasing hormone and measures the amount of testosterone produced in response.
  • Dustin Reichard, Nicole Gerlach (2013-03-29)
    Directions for making subcutaneous hormone implants (crystalline testosterone in Silastic tubing).
  • Jen Grindstaff (2013-03-29)
    Protocol for an enzyme-linked immunosorbent assay (ELISA) which gives a quantitative measure of total IgG levels in plasma from Japanese quail.
  • Ian Parker-Renga (2013-03-29)
    An ethogram describing various categories of observed behavior, specific for female juncos.
  • Jodie Jawor, Danielle Whittaker, Nicole Gerlach, Sarah Wanamaker (2013-03-29)
    Standard Operating Procedure for storing and using regulated substances (steroid hormones).
  • Danielle Whittaker, Nicole Gerlach (2013-03-29)
    Protocol for PCR of extracted avian DNA. Includes directions for PCR to amplify microsatellite loci (used for paternity analysis) and for PCR to amplify sex-specific loci (used for determining sample sex). Includes ...
  • Danielle Whittaker (2013-03-29)
    Protocol for PCR of extracted avian DNA using primers that can determine the sex of the samples. Uses agarose gels to determine the amplified alleles.
  • Christine Bergeon Burns (2013-03-29)
    Recipe for mixing a solution of lutenizing hormone (LH) from a purchased powder.
  • Joe Casto, Nicole Gerlach (2013-03-29)
    Protocol for administering a PHA immune challenge to nestlings and adults.
  • Danielle Whittaker (2013-03-29)
    Questions about methodology for collecting preen oil samples from dark-eyed juncos.
  • Nicole Gerlach (2013-03-29)
    Protocol for extracting DNA from whole blood stored in lysis buffer. Uses IBI Scientific MINI Genomic DNA extraction kits. Includes checklist for each step.
  • Danielle Whittaker (2013-03-29)
    Protocol for extracting DNA from whole blood stored in lysis buffer. Uses the phenol-chloroform method of extraction.
  • Nicole Gerlach (2013-03-29)
    Protocol for extracting DNA from whole blood stored in lysis buffer. Uses the phenol-chloroform method of extraction. Modified from full protocol to include checklist for each step.
  • Joe Lipar (2013-03-29)
    This protocol can be used to measure the concentration of steroid hormones in yolk samples. This protocol has been copied and modified from protocols provided by Dr. John Wingfield at the University of Washington and Dr. ...

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