Browsing Lab Protocols by Title

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  • Ketterson/Nolan research group (2013-03-29)
    Protocol for collecting blood samples for later DNA extraction
  • Christine Bergeon Burns, Kimberly Rosvall (2013-03-29)
    Protocol for reverse transcriptase / cDNA synthesis from extracted RNA samples.
  • Joe Casto, Lynn Siefferman, Christine Bergeon Burns (2013-03-29)
    Recipe for mixing a solution of gonadotropin releasing hormone (c-GnRH) from a powder form.
  • Kimberly Rosvall, Dustin Reichard (2013-03-29)
    Protocol for corticosterone EIA, based on the CORT kit from Caymen
  • Jen Grindstaff (2013-03-29)
    Protocol to prepare frozen egg yolks for use in a variety of enzyme-linked immunosorbent assays (ELISAs).
  • Unsure original creator, possibly updated by Kristal Cain? (2013-03-29)
    Protocol to prepare frozen egg yolks for use in a variety of laboratory tests (ELIZA, Hormone assay, Carotenoid HPLC)
  • Steve Schoech (2013-03-29)
    A spreadsheet for calculating steroid hormone concentrations from radioimmunoassay results. Calculates concentrations of progesterone, testosterone, estradiol, corticosterone, and dihydrotestosterone.
  • Joe Casto, Joel McGlothlin, Ellen Ketterson (2013-03-29)
    Protocol for administering a GnRH challenge to a songbird. This procedure injects a bird with a known concentration of gonadotropin releasing hormone and measures the amount of testosterone produced in response.
  • Dustin Reichard, Nicole Gerlach (2013-03-29)
    Directions for making subcutaneous hormone implants (crystalline testosterone in Silastic tubing).
  • Jen Grindstaff (2013-03-29)
    Protocol for an enzyme-linked immunosorbent assay (ELISA) which gives a quantitative measure of total IgG levels in plasma from Japanese quail.
  • Ian Parker-Renga (2013-03-29)
    An ethogram describing various categories of observed behavior, specific for female juncos.
  • Jodie Jawor, Danielle Whittaker, Nicole Gerlach, Sarah Wanamaker (2013-03-29)
    Standard Operating Procedure for storing and using regulated substances (steroid hormones).
  • Danielle Whittaker, Nicole Gerlach (2013-03-29)
    Protocol for PCR of extracted avian DNA. Includes directions for PCR to amplify microsatellite loci (used for paternity analysis) and for PCR to amplify sex-specific loci (used for determining sample sex). Includes ...
  • Danielle Whittaker (2013-03-29)
    Protocol for PCR of extracted avian DNA using primers that can determine the sex of the samples. Uses agarose gels to determine the amplified alleles.
  • Christine Bergeon Burns (2013-03-29)
    Recipe for mixing a solution of lutenizing hormone (LH) from a purchased powder.
  • Joe Casto, Nicole Gerlach (2013-03-29)
    Protocol for administering a PHA immune challenge to nestlings and adults.
  • Danielle Whittaker (2013-03-29)
    Questions about methodology for collecting preen oil samples from dark-eyed juncos.
  • Nicole Gerlach (2013-03-29)
    Protocol for extracting DNA from whole blood stored in lysis buffer. Uses IBI Scientific MINI Genomic DNA extraction kits. Includes checklist for each step.
  • Danielle Whittaker (2013-03-29)
    Protocol for extracting DNA from whole blood stored in lysis buffer. Uses the phenol-chloroform method of extraction.
  • Nicole Gerlach (2013-03-29)
    Protocol for extracting DNA from whole blood stored in lysis buffer. Uses the phenol-chloroform method of extraction. Modified from full protocol to include checklist for each step.

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