Show simple item record Chen, Lingling Ramsey, Andrew Zheng, Zhida Li, Yali 2012-04-02T17:06:15Z 2012-04-02T17:06:15Z 2010-12
dc.identifier.citation Yali Li, Zhida Zheng, Andrew Ramsey, and Lingling Chen. Analysis of Peptides and Proteins in Their Binding to GroEL, J. Pept. Sci. 16, 12: 693-700, 2010 en
dc.identifier.uri en
dc.description.abstract The GroEL-GroES is an essential molecular chaperon system that assists protein folding in cell. Binding of various substrate proteins to GroEL is one of the key aspects in GroEL-assisted protein folding. Small peptides may mimic segments of the substrate proteins in contact with GroEL, and allow detailed structural analysis of the interactions. A model peptide SBP has been shown to bind to a region in GroEL that is important for binding of substrate proteins. Here, we investigated whether the observed GroEL-SBP interaction represented those of GroEL-substrate proteins, and whether SBP was able to mimic various aspects of substrate proteins in GroE- assisted protein folding cycle. We found that SBP competed with substrate proteins, including α-lactalbumin, rhodanese, and malate dehydrogenase, in binding to GroEL. SBP stimulated GroEL ATP hydrolysis rate in a manner similar to that of α-lactalbumin. SBP did not prevent GroES from binding to GroEL, and GroES association reduced the ATPase rates of GroEL/SBP and GroEL/α-lactalbumin to a comparable extent. Binding of both SBP and α-lactalbumin to apo GroEL was dominated by hydrophobic interaction. Interestingly, association of α- lactalbumin to GroEL/GroES was thermodynamically distinct from that to GroEL with reduced affinity and decreased contribution from hydrophobic interaction. However, SBP did not display such differential binding behaviors to apo GroEL and GroEL/GroES, likely due to the lack of a contiguous polypeptide chain that links all of the bound peptide fragments. Nevertheless, studies using peptides provide valuable information on the nature of GroEL-substrate protein interaction, which is central to understand the mechanism of GroEL-assisted protein folding. en
dc.language.iso en_US en
dc.publisher European Peptide Society and John Wiley & Sons, Ltd. en
dc.rights Copyright © 2010 European Peptide Society and John Wiley & Sons, Ltd. en
dc.subject molecular chaperon en
dc.subject substrate folding en
dc.subject ITC en
dc.subject peptide en
dc.subject GroEL en
dc.title Analysis of Peptides and Proteins in Their Binding to GroEL en
dc.type Article en

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