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dc.contributor.author Tang, Haixu
dc.contributor.author Lynch, Michael
dc.contributor.author Kim, Sun
dc.contributor.author Gao, Xiang
dc.contributor.author Schaack, Sarah
dc.contributor.author Rho, Mina
dc.date.accessioned 2012-01-03T22:19:56Z
dc.date.available 2012-01-03T22:19:56Z
dc.date.issued 2010-07-09
dc.identifier.citation Rho, M., Schaack, S., Gao, X., Kim, S., Lynch, M., & Tang, H. (2010). LTR retroelements in the genome of daphnia pulex. BMC Genomics, 11(1). http://dx.doi.org/10.1186/1471-2164-11-425 en
dc.identifier.uri http://dx.doi.org/10.1186/1471-2164-11-425 en
dc.identifier.uri http://hdl.handle.net/2022/14036
dc.description.abstract Background: Long terminal repeat (LTR) retroelements represent a successful group of transposable elements (TEs)that have played an important role in shaping the structure of many eukaryotic genomes. Here, we present a genomewide analysis of LTR retroelements in Daphnia pulex, a cyclical parthenogen and the first crustacean for which the whole genomic sequence is available. In addition, we analyze transcriptional data and perform transposon display assays of lab-reared lineages and natural isolates to identify potential influences on TE mobility and differences in LTR retroelements loads among individuals reproducing with and without sex. Results: We conducted a comprehensive de novo search for LTR retroelements and identified 333 intact LTR retroelements representing 142 families in the D. pulex genome. While nearly half of the identified LTR retroelements belong to the gypsy group, we also found copia (95), BEL/Pao (66) and DIRS (19) retroelements. Phylogenetic analysis of reverse transcriptase sequences showed that LTR retroelements in the D. pulex genome form many lineages distinct from known families, suggesting that the majority are novel. Our investigation of transcriptional activity of LTR retroelements using tiling array data obtained from three different experimental conditions found that 71 LTR retroelements are actively transcribed. Transposon display assays of mutation-accumulation lines showed evidence for putative somatic insertions for two DIRS retroelement families. Losses of presumably heterozygous insertions were observed in lineages in which selfing occurred, but never in asexuals, highlighting the potential impact of reproductive mode on TE abundance and distribution over time. The same two families were also assayed across natural isolates (both cyclical parthenogens and obligate asexuals) and there were more retroelements in populations capable of reproducing sexually for one of the two families assayed. Conclusions: Given the importance of LTR retroelements activity in the evolution of other genomes, this comprehensive survey provides insight into the potential impact of LTR retroelements on the genome of D. pulex, a cyclically parthenogenetic microcrustacean that has served as an ecological model for over a century. en
dc.language.iso en_US en
dc.publisher BioMed Central en
dc.rights Copyright 2010, BioMed Central Ltd. en
dc.title LTR retroelements in the genome of Daphnia pulex en
dc.type Article en


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