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Browsing Biology by Type "Molecular Protocols"

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Now showing 1 - 11 of 11
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    Blood Sample Collection for DNA
    (2013-03-29) Ketterson/Nolan research group
    Protocol for collecting blood samples for later DNA extraction
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    cDNA synthesis protocol
    (2013-03-29) Christine Bergeon Burns, Kimberly Rosvall
    Protocol for reverse transcriptase / cDNA synthesis from extracted RNA samples.
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    Microsatellite PCR and ABI3730 Genotyping Instructions
    (2013-03-29) Danielle Whittaker, Nicole Gerlach
    Protocol for PCR of extracted avian DNA. Includes directions for PCR to amplify microsatellite loci (used for paternity analysis) and for PCR to amplify sex-specific loci (used for determining sample sex). Includes directions for PCR reactions as well as analysis of PCR product on the ABI3730. Also includes information on the primers used by the Ketterson lab for analyzing junco microsatellite loci.
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    Molecular sexing of birds
    (2013-03-29) Danielle Whittaker
    Protocol for PCR of extracted avian DNA using primers that can determine the sex of the samples. Uses agarose gels to determine the amplified alleles.
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    Protocol for kit extraction of DNA from whole blood samples
    (2013-03-29) Nicole Gerlach
    Protocol for extracting DNA from whole blood stored in lysis buffer. Uses IBI Scientific MINI Genomic DNA extraction kits. Includes checklist for each step.
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    Protocol for phenol-chloroform extraction of DNA from whole blood samples
    (2013-03-29) Danielle Whittaker
    Protocol for extracting DNA from whole blood stored in lysis buffer. Uses the phenol-chloroform method of extraction.
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    Protocol for phenol-chloroform extraction of DNA from whole blood samples, with checklist
    (2013-03-29) Nicole Gerlach
    Protocol for extracting DNA from whole blood stored in lysis buffer. Uses the phenol-chloroform method of extraction. Modified from full protocol to include checklist for each step.
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    qPCR protocol
    (2013-03-29) Christine Bergeon Burns, Kimberly Rosvall
    Protocol for quantitative PCR to analyse RNA concentration from cDNA.
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    Recipe for Longmire's solution
    (2013-03-29) Ketterson/Nolan research group, Sara Schrock
    Recipe for making Longmire's solution, a cell lysis buffer for storing blood samples for DNA analysis. Also includes recipes for stock solutions used in making Longmire's solution.
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    RNA extraction protocols
    (2013-03-29) Christine Bergeon Burns, Kimberly Rosvall
    Protocol for extraction of RNA from avian tissue using Trisol and includes instructions for gel electrophoresis.
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    Salt extraction of DNA from whole blood
    (2013-03-29) From Peter Dunn's lab; unsure if updated by Kettlab members
    Protocol for extracting DNA from whole blood stored in lysis buffer. Uses the salt solution extraction buffer.