Biology
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Item Item Raw microscopy data for Wolbachia infection of JW18 cellsNewton, IreneWolbachia pipientis is an incredibly widespread bacterial symbiont of insects, present in an estimated 25-52% of species worldwide. Wolbachia is faithfully maternally transmitted both in a laboratory setting and in the wild. In an established infection, Wolbachia is primarily intracellular, residing within host-derived vacuoles that are associated with the endoplasmic reticulum. However, Wolbachia also frequently transfer between host species, requiring an extracellular stage to their life cycle. Indeed, Wolbachia has been moved between insect species for the precise goal of controlling populations. The use of Wolbachia in this application requires we better understand how it initiates and establishes new infections. Here we designed a novel method for live-tracking Wolbachia during infection using a combination of stains and microscopy. We show that live Wolbachia are taken up by host cells at a much faster rate than dead Wolbachia cells, indicating that Wolbachia play a role in their own uptake and that Wolbachia colonization is not just a passive process. We also show that the host actin cytoskeleton must be intact for this to occur, and that drugs that disrupt the actin cytoskeleton effectively abrogate Wolbachia uptake. The development of this live infection assay will assist in future efforts to characterize Wolbachia factors used during host infection.Item Team photo 1991(1991) Ketterson, EllenItem Team photo 1997(1997) Ketterson, EllenFront row (from left): Joe Casto, Brandi Vanroo, Tom Smulders middle row: Kerry Jones, Erin Kennedy Back row: Jennifer Mikesell-Zaebst, Eric Snajdr, Ellen Ketterson, David Aylor.Item Team photo 1998(1998) Ketterson, EllenFront row (from left): Ellen Ketterson, Jennifer Klefner, Jennifer Grindstaff, Michelle Hill, David Ziolkowski, Val Nolan Jr. Back row: Elise Donnelly, Barndi VanRoo, Carol Aron, Eric Snajdr, Diane NeudorfItem Team photo 1999(1999) Ketterson, EllenFrom left: Ethan Clotfelfter, David Ziolkowski, Joe Casto, Diane Neudorf, Val Nolan Jr. Dafna Hoppenstand, Erin Hagen, Bill Garrison, Eric Snajdr, Ellen KettersonItem Team photo 2000(2000) Ketterson, EllenFront row (from left): Ellen Ketterson, Val Nolan Jr., Kristin Schubert, Diane Neudorf, Ethan Clotfelter. Back row: Eric Snajdr, Ian Paker-Renga, Joel McGlothlin, Amy Clotfelter. Not pictured: Joe Casto, Eva Sanders Allen,Item Team photo 2002(2002) Ketterson, EllenFrom left: Jackie Gaudioso, Wendy Wolf, Eric Snajdr, Dawn O'Neal, Katie Hill. Not pictured: Deb Duffy, Ethan Clotfelter, Ian Parker-Renga, Ellen Ketterson, Val Nolan Jr.Item Team photo 2004(2004) Ketterson, EllenFrom left: Sara Schrock, Eric Snajdr, Jackie Gaudioso, Tim Greives, Joel McGlothlin. Not pictured: Ellen Ketterson, Val Nolan Jr.Item Team photo 2005(2005) Ketterson, EllenFront row (from left): Nicki Gerlach, Jenny Phillips, Dawn OʼNeal, Andrea (Annie) Lindsey, Katie Pavlis. Back row: Joel McGlothlin, Peter Stevens, Eric Snajdr Not pictured: Jodie Jawor, Ellen Ketterson, Val Nolan Jr.Item Team photo 2005 II(2005) Ketterson, Ellenfrom left: Peter Stevens, Dawn OʼNeal, Andrea (Annie) Lindsey, Nicki Gerlach, Jenny Phillips, Katie Pavlis, Eric Snajdr, Joel McGlothlin. Not pictured: Jodie Jawor, Ellen Ketterson, Val Nolan Jr.Item Team photo 2006(2006) Ketterson, EllenFrom left: Eric Snajdr, Krystle Ainsworth, Amanda Bessler, Becky Houdeck, Dawn O’Neal, Sara Schrock, Dustin Reichard. Not pictured: Joel McGlothlin, Jodie Jawor, Carrington Knox, Ellen Ketterson, Val Nolan