Chemistry
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Item Proteomic analysis of the Caulobacter crescentus stalk indicates competence for nutrient uptake(Molecular Microbiology, 2002-08-15) Ireland, Marcia M. E.; Karty, Jonathan; Quardokus, Ellen M.; Reilly, James P.; Brun, Yves V.Caulobacter crescentus , a Gram-negative α -purple proteobacterium, is an oligotroph that lives in aquatic environments dilute in nutrients. This bacterium divides asymmetrically. Part of this asymmetric cell division involves the formation of a prosthecum at one pole, referred to as the stalk, which replaces the flagellum of the motile swarmer cell. Little is known about the synthesis or function of the stalk. The stalk is an extension of the cell membranes and peptidoglycan layer, and stalk elongation is stimulated by phosphate starvation. In this study, we have taken advantage of two-dimensional gel (2D gel) electro-phoresis as well as the fully sequenced genome of Caulobacter to study the proteome of the stalk. We modified a stalk-shedding mutant strain of Caulobacter crescentus to increase the yield of stalk material shed and performed 2D gel electrophoresis of purified stalks and cellular fractions. Comparison of the stalk 2D gel with the 2D gels of cell membrane and soluble fractions showed that the stalk is mostly free of cytoplasmic proteins and has a profile very similar to that of the cell membrane. Of the 172 proteins on a stalk 2D gel, we report the identification of 64 spots, corresponding to 39 different proteins present in the stalk of Caulobacter. The identifications include several TonB-dependent receptors, two OmpA family proteins, a dipeptidase, GlpQ, two alkaline phosphatases, 3-phytase, a putative TolC protein and 11 proteins of unknown function. These identifications are consistent with the hypothesis that the stalk plays a role in nutrient uptake.Item Lesions in Phycoerythrin Chromophore Biosynthesis in Fremyella diplosiphon Reveal Coordinated Light Regulation of Apoprotein and Pigment Biosynthetic Enzyme Gene Expression(The Plant Cell, 2003) Alvey, Richard M.; Karty, Jonathan; Roos, Elicia; Reilly, James P.; Kehoe, David M.We have characterized the regulation of the expression of the pebAB operon, which encodes the enzymes required for phycoerythrobilin synthesis in the filamentous cyanobacterium Fremyella diplosiphon. The expression of the pebAB operon was found to be regulated during complementary chromatic adaptation, the system that controls the light responsiveness of genes that encode several light-harvesting proteins in F. diplosiphon. Our analyses of pebA mutants demonstrated that although the levels of phycoerythrin and its associated linker proteins decreased in the absence of phycoerythrobilin, there was no significant modulation of the expression of pebAB and the genes that encode phycoerythrin. Instead, regulation of the expression of these genes is coordinated at the level of RNA accumulation by the recently discovered activator CpeR.Item Towards Local Probe Vibrational Spectroscopy of Single Virus Particles(2004) DuFort, ChristopherItem Synthesis of cyclic Ethers via Lewis Acid Mediated Oxepane Formation(2004) Singh, AnandItem Table of Contents G-M(2004) Indiana University, Department of ChemistryItem Regioselective Rhodium-Catalyzed Allylic Amination(2004) Clizbe, ElizabethItem Synthesis and Photostability Studies of Functionalized Luminescent Semiconductor Particles(2004) Goicochea, NancyItem Mapping the Light Intensity Transmitted through Nanoscale Apertures(2004) Rawlinson, Nathan D.Item Novel Designs for Ion Mobility Spectrometry/Time-of-Flight Mass Spectrometry(2004) Merrenbloom, Samuel I.Item Steps Toward Peptide Polymerization in Polyacrylamide Gels(2004) Copes, AshleyItem Regioselective Rhodium-Catalyzed Allylic Etherfication(2004) Cui, XiaoItem VirusX: Simulation of Viral Conformational Changes(2004) Miao, YinglongItem Synthesis of C(5-)C(18)ansa-Chain Intermediate Toward the Total Synthesis of Kendomycin(2004) Opsitnick, ElizabethItem Table of Contents A-F(2004) Indiana University, Department of ChemistryItem Toward the Total Synthesis of Tubelactomicin A(2004) Chow, NicholasItem Table of Contents N-Z(2004) Indiana University, Department of Chemistry