2024-03-28T19:29:52Zhttps://scholarworks.iu.edu/dspace-oai/requestoai:scholarworks.iu.edu:2022/153852021-10-19T00:00:57Zcom_2022_15252com_2022_7911com_2022_7910com_2022_19675com_2022_19673col_2022_15255
How to Make Hormone Implants
Dustin Reichard, Nicole Gerlach
hormones, testosterone, implants, endocrinology, birds, field
Directions for making subcutaneous hormone implants (crystalline testosterone in Silastic tubing).
2013-03-29T15:02:01Z
2013-03-29T15:02:01Z
2013-03-29
Androgens
http://hdl.handle.net/2022/15385
By downloading this document or using any information contained therein, you agree to the Creative Commons Attribution-NonCommercial-ShareAlike (CC BY-NC-SA) license terms, which explain terms governing use, creation of derivative research, and requirements for citing the document.
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2012
oai:scholarworks.iu.edu:2022/153862021-10-19T00:01:43Zcom_2022_15252com_2022_7911com_2022_7910com_2022_19675com_2022_19673col_2022_15255
Ketterson Lab Androgen Standard Operating Procedure
Jodie Jawor, Danielle Whittaker, Nicole Gerlach, Sarah Wanamaker
hormones, lab, testosterone, endocrinology, policy
Standard Operating Procedure for storing and using regulated substances (steroid hormones).
2013-03-29T15:02:01Z
2013-03-29T15:02:01Z
2013-03-29
Androgens
http://hdl.handle.net/2022/15386
By downloading this document or using any information contained therein, you agree to the Creative Commons Attribution-NonCommercial-ShareAlike (CC BY-NC-SA) license terms, which explain terms governing use, creation of derivative research, and requirements for citing the document.
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2000-2012
oai:scholarworks.iu.edu:2022/153872021-10-19T00:01:42Zcom_2022_15252com_2022_7911com_2022_7910com_2022_19675com_2022_19673col_2022_15255
Subcutaneous Implant Methods
Nicole Gerlach, Kimberly Rosvall
hormones, implants, testosterone, surgery, anesthetic
Protocols for administering subcutaneous silastic hormone implants. Includes descriptions of the surgical method, including protocols for using inhaled anesthetics, and the non-surgical trochar implantation method.
2013-03-29T15:02:02Z
2013-03-29T15:02:02Z
2013-03-29
Androgens
http://hdl.handle.net/2022/15387
By downloading this document or using any information contained therein, you agree to the Creative Commons Attribution-NonCommercial-ShareAlike (CC BY-NC-SA) license terms, which explain terms governing use, creation of derivative research, and requirements for citing the document.
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2006-2010
oai:scholarworks.iu.edu:2022/153882021-10-19T00:01:46Zcom_2022_15252com_2022_7911com_2022_7910com_2022_19675com_2022_19673col_2022_15255
CORT EIA protocol
Kimberly Rosvall, Dustin Reichard
hormones, endocrinology, corticosterone, cort, assay, lab, EIA, plasma
Protocol for corticosterone EIA, based on the CORT kit from Caymen
2013-03-29T15:02:02Z
2013-03-29T15:02:02Z
2013-03-29
Enzyme Immunoassay Protocol
http://hdl.handle.net/2022/15388
By downloading this document or using any information contained therein, you agree to the Creative Commons Attribution-NonCommercial-ShareAlike (CC BY-NC-SA) license terms, which explain terms governing use, creation of derivative research, and requirements for citing the document.
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2011
oai:scholarworks.iu.edu:2022/153892021-10-19T00:01:49Zcom_2022_15252com_2022_7911com_2022_7910com_2022_19675com_2022_19673col_2022_15255
Protocol For Using Assay Designs� Testosterone EIA Kit With Bird Plasma without radio-labeled testosterone in samples
Nicole Gerlach
hormones, endocrinology, testosterone, plasma, assay, EIA, lab
Protocol for testosterone EIA, based on the protocol with the kit from Assay Designs. Similar to the full protocol, but modified so that radiolabeled testosterone is only added to the standards, not the samples.
2013-03-29T15:02:02Z
2013-03-29T15:02:02Z
2013-03-29
Enzyme Immunoassay Protocol
http://hdl.handle.net/2022/15389
By downloading this document or using any information contained therein, you agree to the Creative Commons Attribution-NonCommercial-ShareAlike (CC BY-NC-SA) license terms, which explain terms governing use, creation of derivative research, and requirements for citing the document.
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2005
oai:scholarworks.iu.edu:2022/153902021-10-19T00:05:54Zcom_2022_15252com_2022_7911com_2022_7910com_2022_19675com_2022_19673col_2022_15255
Protocol For Using Assay Designs� Testosterone EIA Kit With Bird Plasma
Danielle Whittaker
hormones, endocrinology, testosterone, plasma, assay, EIA, lab
Protocol for testosterone EIA, based on the protocol with the kit from Assay Designs
2013-03-29T15:02:02Z
2013-03-29T15:02:02Z
2013-03-29
Enzyme Immunoassay Protocol
http://hdl.handle.net/2022/15390
By downloading this document or using any information contained therein, you agree to the Creative Commons Attribution-NonCommercial-ShareAlike (CC BY-NC-SA) license terms, which explain terms governing use, creation of derivative research, and requirements for citing the document.
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
2006
oai:scholarworks.iu.edu:2022/153912014-01-31T20:04:07Zcom_2022_15252com_2022_7911com_2022_7910com_2022_19675com_2022_19673col_2022_15255
Testosterone EIA Calculations Spreadsheet
Joe Casto, Nicole Gerlach, Ketterson/Nolan research group
hormones, endocrinology, testosterone, plasma, assay, EIA, lab, calculations
A spreadsheet for calculating testosterone concentrations based on enzyme immunoassay results. Has spaces for the radiation counts per minute to be entered to calculate recovery rates, the concentration from the plate reader, and the amount of plasma, and calculates the pg/mL and ng/mL of testosterone in each sample. Includes sheets for 5 assays, and calculations for comparing standards across plates and calculating corrected concentration values.
2013-03-29T15:02:02Z
2013-03-29T15:02:02Z
2013-03-29
Enzyme Immunoassay Protocol
http://hdl.handle.net/2022/15391
By downloading this document or using any information contained therein, you agree to the Creative Commons Attribution-NonCommercial-ShareAlike (CC BY-NC-SA) license terms, which explain terms governing use, creation of derivative research, and requirements for citing the document.
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2000-2012
oai:scholarworks.iu.edu:2022/153922021-10-19T00:06:16Zcom_2022_15252com_2022_7911com_2022_7910com_2022_19675com_2022_19673col_2022_15255
Adrenocorticotropic Hormone (ACTH) Challenge Protocol
Nicole Gerlach
hormones, challenge, protocol, ACTH, HPA axis, injection, endocrinology
Recipe for mixing a solution of adrenocorticotropic hormone (ACTH), and protocol for giving ACTH injections as part of an ACTH challenge
2013-03-29T15:02:02Z
2013-03-29T15:02:02Z
2013-03-29
Hormone Challenge
http://hdl.handle.net/2022/15392
By downloading this document or using any information contained therein, you agree to the Creative Commons Attribution-NonCommercial-ShareAlike (CC BY-NC-SA) license terms, which explain terms governing use, creation of derivative research, and requirements for citing the document.
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2004
oai:scholarworks.iu.edu:2022/153932021-10-18T09:47:20Zcom_2022_15252com_2022_7911com_2022_7910com_2022_19675com_2022_19673col_2022_15255
c-GnRH solution recipe
Joe Casto, Lynn Siefferman, Christine Bergeon Burns
hormones, challenge, recipe, GnRH, HPG axis, endocrinology
Recipe for mixing a solution of gonadotropin releasing hormone (c-GnRH) from a powder form.
2013-03-29T15:02:02Z
2013-03-29T15:02:02Z
2013-03-29
Hormone Challenge
http://hdl.handle.net/2022/15393
By downloading this document or using any information contained therein, you agree to the Creative Commons Attribution-NonCommercial-ShareAlike (CC BY-NC-SA) license terms, which explain terms governing use, creation of derivative research, and requirements for citing the document.
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2003-2012
oai:scholarworks.iu.edu:2022/153942021-10-18T09:49:49Zcom_2022_15252com_2022_7911com_2022_7910com_2022_19675com_2022_19673col_2022_15255
GnRH challenge protocol
Joe Casto, Joel McGlothlin, Ellen Ketterson
hormones, challenge, protocol, GnRH, HPG axis, injection, endocrinology
Protocol for administering a GnRH challenge to a songbird. This procedure injects a bird with a known concentration of gonadotropin releasing hormone and measures the amount of testosterone produced in response.
2013-03-29T15:02:03Z
2013-03-29T15:02:03Z
2013-03-29
Hormone Challenge
http://hdl.handle.net/2022/15394
By downloading this document or using any information contained therein, you agree to the Creative Commons Attribution-NonCommercial-ShareAlike (CC BY-NC-SA) license terms, which explain terms governing use, creation of derivative research, and requirements for citing the document.
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2003-2004
oai:scholarworks.iu.edu:2022/153952021-10-18T09:49:50Zcom_2022_15252com_2022_7911com_2022_7910com_2022_19675com_2022_19673col_2022_15255
o-LH solution recipe
Christine Bergeon Burns
recipe, LH, HPA axis, hormones, endocrinology
Recipe for mixing a solution of lutenizing hormone (LH) from a purchased powder.
2013-03-29T15:02:03Z
2013-03-29T15:02:03Z
2013-03-29
Hormone Challenge
http://hdl.handle.net/2022/15395
By downloading this document or using any information contained therein, you agree to the Creative Commons Attribution-NonCommercial-ShareAlike (CC BY-NC-SA) license terms, which explain terms governing use, creation of derivative research, and requirements for citing the document.
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
2010
oai:scholarworks.iu.edu:2022/153962021-10-18T08:49:40Zcom_2022_15252com_2022_7911com_2022_7910com_2022_19675com_2022_19673col_2022_15255
Egg Yolk ELISA preparation
Jen Grindstaff
egg, egg yolk, assays, immune, lab
Protocol to prepare frozen egg yolks for use in a variety of enzyme-linked immunosorbent assays (ELISAs).
2013-03-29T15:02:03Z
2013-03-29T15:02:03Z
2013-03-29
Immune
http://hdl.handle.net/2022/15396
By downloading this document or using any information contained therein, you agree to the Creative Commons Attribution-NonCommercial-ShareAlike (CC BY-NC-SA) license terms, which explain terms governing use, creation of derivative research, and requirements for citing the document.
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2002
oai:scholarworks.iu.edu:2022/153972021-10-18T09:49:51Zcom_2022_15252com_2022_7911com_2022_7910com_2022_19675com_2022_19673col_2022_15255
IgG ELISA protocol
Jen Grindstaff
immune, plasma, assay, lab
Protocol for an enzyme-linked immunosorbent assay (ELISA) which gives a quantitative measure of total IgG levels in plasma from
Japanese quail.
2013-03-29T15:02:03Z
2013-03-29T15:02:03Z
2013-03-29
Immune
http://hdl.handle.net/2022/15397
By downloading this document or using any information contained therein, you agree to the Creative Commons Attribution-NonCommercial-ShareAlike (CC BY-NC-SA) license terms, which explain terms governing use, creation of derivative research, and requirements for citing the document.
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2002
oai:scholarworks.iu.edu:2022/153982021-10-18T09:49:52Zcom_2022_15252com_2022_7911com_2022_7910com_2022_19675com_2022_19673col_2022_15255
Phytohemaglutinin (PHA) Immune Challenge protocol
Joe Casto, Nicole Gerlach
immune, nestlings, photos, injection, PHA, challenge
Protocol for administering a PHA immune challenge to nestlings and adults.
2013-03-29T15:02:03Z
2013-03-29T15:02:03Z
2013-03-29
Immune
http://hdl.handle.net/2022/15398
By downloading this document or using any information contained therein, you agree to the Creative Commons Attribution-NonCommercial-ShareAlike (CC BY-NC-SA) license terms, which explain terms governing use, creation of derivative research, and requirements for citing the document.
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2001-2006
oai:scholarworks.iu.edu:2022/153992021-10-19T00:08:02Zcom_2022_15252com_2022_7911com_2022_7910com_2022_19675com_2022_19673col_2022_15255
Sheep Red Blood Cell Immune Assay
Devin Zysling
immune, SRBC
Protocol for measuring immune response to injection of sheep red blood cells (SRBC)
2013-03-29T15:02:03Z
2013-03-29T15:02:03Z
2013-03-29
Immune
http://hdl.handle.net/2022/15399
By downloading this document or using any information contained therein, you agree to the Creative Commons Attribution-NonCommercial-ShareAlike (CC BY-NC-SA) license terms, which explain terms governing use, creation of derivative research, and requirements for citing the document.
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2005?
oai:scholarworks.iu.edu:2022/154002021-10-18T09:49:55Zcom_2022_15252com_2022_7911com_2022_7910com_2022_19675com_2022_19673col_2022_15255
cDNA synthesis protocol
Christine Bergeon Burns, Kimberly Rosvall
protocol, RNA, cDNA, DNA, genotyping, gene expression
Protocol for reverse transcriptase / cDNA synthesis from extracted RNA samples.
2013-03-29T15:02:03Z
2013-03-29T15:02:03Z
2013-03-29
Molecular Protocols
http://hdl.handle.net/2022/15400
By downloading this document or using any information contained therein, you agree to the Creative Commons Attribution-NonCommercial-ShareAlike (CC BY-NC-SA) license terms, which explain terms governing use, creation of derivative research, and requirements for citing the document.
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
2010
oai:scholarworks.iu.edu:2022/154012021-10-18T09:50:56Zcom_2022_15252com_2022_7911com_2022_7910com_2022_19675com_2022_19673col_2022_15255
Protocol for kit extraction of DNA from whole blood samples
Nicole Gerlach
protocol, DNA, extraction, blood, lab
Protocol for extracting DNA from whole blood stored in lysis buffer. Uses IBI Scientific MINI Genomic DNA extraction kits. Includes checklist for each step.
2013-03-29T15:02:04Z
2013-03-29T15:02:04Z
2013-03-29
Molecular Protocols
http://hdl.handle.net/2022/15401
By downloading this document or using any information contained therein, you agree to the Creative Commons Attribution-NonCommercial-ShareAlike (CC BY-NC-SA) license terms, which explain terms governing use, creation of derivative research, and requirements for citing the document.
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
2011
oai:scholarworks.iu.edu:2022/154022021-10-19T00:08:05Zcom_2022_15252com_2022_7911com_2022_7910com_2022_19675com_2022_19673col_2022_15255
Blood Sample Collection for DNA
Ketterson/Nolan research group
DNA, blood, field
Protocol for collecting blood samples for later DNA extraction
2013-03-29T15:02:04Z
2013-03-29T15:02:04Z
2013-03-29
Molecular Protocols
http://hdl.handle.net/2022/15402
By downloading this document or using any information contained therein, you agree to the Creative Commons Attribution-NonCommercial-ShareAlike (CC BY-NC-SA) license terms, which explain terms governing use, creation of derivative research, and requirements for citing the document.
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
2000
oai:scholarworks.iu.edu:2022/154032021-10-18T09:51:14Zcom_2022_15252com_2022_7911com_2022_7910com_2022_19675com_2022_19673col_2022_15255
Microsatellite PCR and ABI3730 Genotyping Instructions
Danielle Whittaker, Nicole Gerlach
protocol, DNA, PCR, birds, sex, genotyping, microsatellites, lab
Protocol for PCR of extracted avian DNA. Includes directions for PCR to amplify microsatellite loci (used for paternity analysis) and for PCR to amplify sex-specific loci (used for determining sample sex). Includes directions for PCR reactions as well as analysis of PCR product on the ABI3730. Also includes information on the primers used by the Ketterson lab for analyzing junco microsatellite loci.
2013-03-29T15:02:04Z
2013-03-29T15:02:04Z
2013-03-29
Molecular Protocols
http://hdl.handle.net/2022/15403
By downloading this document or using any information contained therein, you agree to the Creative Commons Attribution-NonCommercial-ShareAlike (CC BY-NC-SA) license terms, which explain terms governing use, creation of derivative research, and requirements for citing the document.
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2008
oai:scholarworks.iu.edu:2022/154042021-10-18T09:51:20Zcom_2022_15252com_2022_7911com_2022_7910com_2022_19675com_2022_19673col_2022_15255
Molecular sexing of birds
Danielle Whittaker
protocol, DNA, PCR, birds, sex, gels, genotyping, lab
Protocol for PCR of extracted avian DNA using primers that can determine the sex of the samples. Uses agarose gels to determine the amplified alleles.
2013-03-29T15:02:04Z
2013-03-29T15:02:04Z
2013-03-29
Molecular Protocols
http://hdl.handle.net/2022/15404
By downloading this document or using any information contained therein, you agree to the Creative Commons Attribution-NonCommercial-ShareAlike (CC BY-NC-SA) license terms, which explain terms governing use, creation of derivative research, and requirements for citing the document.
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
2006
oai:scholarworks.iu.edu:2022/154052021-10-18T09:51:21Zcom_2022_15252com_2022_7911com_2022_7910com_2022_19675com_2022_19673col_2022_15255
Protocol for phenol-chloroform extraction of DNA from whole blood samples
Danielle Whittaker
protocol, DNA, extraction, blood, lab
Protocol for extracting DNA from whole blood stored in lysis buffer. Uses the phenol-chloroform method of extraction.
2013-03-29T15:02:04Z
2013-03-29T15:02:04Z
2013-03-29
Molecular Protocols
http://hdl.handle.net/2022/15405
By downloading this document or using any information contained therein, you agree to the Creative Commons Attribution-NonCommercial-ShareAlike (CC BY-NC-SA) license terms, which explain terms governing use, creation of derivative research, and requirements for citing the document.
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
2006
oai:scholarworks.iu.edu:2022/154062021-10-18T09:52:35Zcom_2022_15252com_2022_7911com_2022_7910com_2022_19675com_2022_19673col_2022_15255
Protocol for phenol-chloroform extraction of DNA from whole blood samples, with checklist
Nicole Gerlach
protocol, DNA, extraction, blood, lab
Protocol for extracting DNA from whole blood stored in lysis buffer. Uses the phenol-chloroform method of extraction. Modified from full protocol to include checklist for each step.
2013-03-29T15:02:04Z
2013-03-29T15:02:04Z
2013-03-29
Molecular Protocols
http://hdl.handle.net/2022/15406
By downloading this document or using any information contained therein, you agree to the Creative Commons Attribution-NonCommercial-ShareAlike (CC BY-NC-SA) license terms, which explain terms governing use, creation of derivative research, and requirements for citing the document.
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
2006
oai:scholarworks.iu.edu:2022/154072021-10-19T00:08:04Zcom_2022_15252com_2022_7911com_2022_7910com_2022_19675com_2022_19673col_2022_15255
qPCR protocol
Christine Bergeon Burns, Kimberly Rosvall
protocol, RNA, cDNA, DNA, genotyping, gene expression, PCR, qPCR
Protocol for quantitative PCR to analyse RNA concentration from cDNA.
2013-03-29T15:02:04Z
2013-03-29T15:02:04Z
2013-03-29
Molecular Protocols
http://hdl.handle.net/2022/15407
By downloading this document or using any information contained therein, you agree to the Creative Commons Attribution-NonCommercial-ShareAlike (CC BY-NC-SA) license terms, which explain terms governing use, creation of derivative research, and requirements for citing the document.
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
2010
oai:scholarworks.iu.edu:2022/154082021-10-19T00:09:52Zcom_2022_15252com_2022_7911com_2022_7910com_2022_19675com_2022_19673col_2022_15255
Recipe for Longmire's solution
Ketterson/Nolan research group, Sara Schrock
recipe, blood, DNA
Recipe for making Longmire's solution, a cell lysis buffer for storing blood samples for DNA analysis. Also includes recipes for stock solutions used in making Longmire's solution.
2013-03-29T15:02:05Z
2013-03-29T15:02:05Z
2013-03-29
Molecular Protocols
http://hdl.handle.net/2022/15408
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1990s, updated 2003-4ish?
oai:scholarworks.iu.edu:2022/154092021-10-19T00:09:55Zcom_2022_15252com_2022_7911com_2022_7910com_2022_19675com_2022_19673col_2022_15255
RNA extraction protocols
Christine Bergeon Burns, Kimberly Rosvall
protocol, RNA, extraction, lab, gels
Protocol for extraction of RNA from avian tissue using Trisol and includes instructions for gel electrophoresis.
2013-03-29T15:02:05Z
2013-03-29T15:02:05Z
2013-03-29
Molecular Protocols
http://hdl.handle.net/2022/15409
By downloading this document or using any information contained therein, you agree to the Creative Commons Attribution-NonCommercial-ShareAlike (CC BY-NC-SA) license terms, which explain terms governing use, creation of derivative research, and requirements for citing the document.
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
2010
oai:scholarworks.iu.edu:2022/154102021-10-19T00:10:00Zcom_2022_15252com_2022_7911com_2022_7910com_2022_19675com_2022_19673col_2022_15255
Salt extraction of DNA from whole blood
From Peter Dunn's lab; unsure if updated by Kettlab members
protocol, DNA, extraction, blood, lab
Protocol for extracting DNA from whole blood stored in lysis buffer. Uses the salt solution extraction buffer.
2013-03-29T15:02:05Z
2013-03-29T15:02:05Z
2013-03-29
Molecular Protocols
http://hdl.handle.net/2022/15410
By downloading this document or using any information contained therein, you agree to the Creative Commons Attribution-NonCommercial-ShareAlike (CC BY-NC-SA) license terms, which explain terms governing use, creation of derivative research, and requirements for citing the document.
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
oai:scholarworks.iu.edu:2022/154112021-10-18T08:49:45Zcom_2022_15252com_2022_7911com_2022_7910com_2022_19675com_2022_19673col_2022_15255
Junco Female Ethogram
Ian Parker-Renga
ethogram, field, behavior, bird
An ethogram describing various categories of observed behavior, specific for female juncos.
2013-03-29T15:02:05Z
2013-03-29T15:02:05Z
2013-03-29
Other
http://hdl.handle.net/2022/15411
By downloading this document or using any information contained therein, you agree to the Creative Commons Attribution-NonCommercial-ShareAlike (CC BY-NC-SA) license terms, which explain terms governing use, creation of derivative research, and requirements for citing the document.
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
2000
oai:scholarworks.iu.edu:2022/154122021-10-18T08:49:47Zcom_2022_15252com_2022_7911com_2022_7910com_2022_19675com_2022_19673col_2022_15255
Preen Oil Collection Questions
Danielle Whittaker
preen oil, field
Questions about methodology for collecting preen oil samples from dark-eyed juncos.
2013-03-29T15:02:05Z
2013-03-29T15:02:05Z
2013-03-29
Other
http://hdl.handle.net/2022/15412
By downloading this document or using any information contained therein, you agree to the Creative Commons Attribution-NonCommercial-ShareAlike (CC BY-NC-SA) license terms, which explain terms governing use, creation of derivative research, and requirements for citing the document.
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2012
oai:scholarworks.iu.edu:2022/154132021-10-19T00:10:01Zcom_2022_15252com_2022_7911com_2022_7910com_2022_19675com_2022_19673col_2022_15255
Spectrometer Protocol
Lynn Siefferman
feather, color, field
Protocol for using the spectrometer to analyze color spectrum from feather samples.
2013-03-29T15:02:05Z
2013-03-29T15:02:05Z
2013-03-29
Other
http://hdl.handle.net/2022/15413
By downloading this document or using any information contained therein, you agree to the Creative Commons Attribution-NonCommercial-ShareAlike (CC BY-NC-SA) license terms, which explain terms governing use, creation of derivative research, and requirements for citing the document.
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2006?
oai:scholarworks.iu.edu:2022/154142021-10-19T00:10:43Zcom_2022_15252com_2022_7911com_2022_7910com_2022_19675com_2022_19673col_2022_15255
Sperm Collecting and Counting Protocol
George Gee, Elaina Tuttle, Tracey Kast
sperm, reproduction, field, microscopy
Protocol for collecting sperm from songbirds, as well as for counting sperm samples. Also includes data sheet.
2013-03-29T15:02:05Z
2013-03-29T15:02:05Z
2013-03-29
Other
http://hdl.handle.net/2022/15414
By downloading this document or using any information contained therein, you agree to the Creative Commons Attribution-NonCommercial-ShareAlike (CC BY-NC-SA) license terms, which explain terms governing use, creation of derivative research, and requirements for citing the document.
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
1994
oai:scholarworks.iu.edu:2022/154152021-10-19T00:11:27Zcom_2022_15252com_2022_7911com_2022_7910com_2022_19675com_2022_19673col_2022_15255
Short protocol for direct radioimmunoassay of corticosterone (a.k.a. Little B)
Joe Casto, Jodie Jawor
hormones, endocrinology, corticosterone, cort, assay, lab, plasma, little b, RIA
This protocol can be used to measure the concentration of corticosterone in small plasma samples. This protocol has been copied and modified from protocols provided by Dr. John Wingfield at the University of Washington. It has been written for use in the laboratory of Dr. Ellen Ketterson at Indiana University, and therefore the details may require modification if the procedure is performed elsewhere. The general procedure, which takes 4 days to complete, is described below. Variations and additional information are provided in the appendices. Prepared October 1999 by Joe Casto, modified October 2003 by Jodie Jawor.
2013-03-29T15:02:05Z
2013-03-29T15:02:05Z
2013-03-29
Radioimmunoassay Protocol
http://hdl.handle.net/2022/15415
By downloading this document or using any information contained therein, you agree to the Creative Commons Attribution-NonCommercial-ShareAlike (CC BY-NC-SA) license terms, which explain terms governing use, creation of derivative research, and requirements for citing the document.
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
1999
oai:scholarworks.iu.edu:2022/154162021-10-18T08:49:49Zcom_2022_15252com_2022_7911com_2022_7910com_2022_19675com_2022_19673col_2022_15255
Egg Yolk preparation
Unsure original creator, possibly updated by Kristal Cain?
egg, egg yolk, assay, hormone, immune, carotenoid, lab
Protocol to prepare frozen egg yolks for use in a variety of laboratory tests (ELIZA, Hormone assay, Carotenoid HPLC)
2013-03-29T15:02:05Z
2013-03-29T15:02:05Z
2013-03-29
Radioimmunoassay Protocol
http://hdl.handle.net/2022/15416
By downloading this document or using any information contained therein, you agree to the Creative Commons Attribution-NonCommercial-ShareAlike (CC BY-NC-SA) license terms, which explain terms governing use, creation of derivative research, and requirements for citing the document.
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
oai:scholarworks.iu.edu:2022/154172021-10-18T08:49:51Zcom_2022_15252com_2022_7911com_2022_7910com_2022_19675com_2022_19673col_2022_15255
Protocol for steroid radioimmunoassay of egg yolks
Joe Lipar
hormones, endocrinology, corticosterone, cort, assay, lab, eggs, egg yolk, testosterone, progesterone, RIA
This protocol can be used to measure the concentration of steroid hormones in yolk samples. This protocol has been copied and modified from protocols provided by Dr. John Wingfield at the University of Washington and Dr. Hubert Schwabl at Washington State University. It has been written for use in the laboratory of Dr. Ellen Ketterson at Indiana University, and therefore the details may require modification if the procedure is performed elsewhere. The general procedure, which takes 5 days to complete, is described below. Variations and additional information are provided in the appendices. Prepared October 1997 by Joe Lipar.
2013-03-29T15:02:05Z
2013-03-29T15:02:05Z
2013-03-29
Radioimmunoassay Protocol
http://hdl.handle.net/2022/15417
By downloading this document or using any information contained therein, you agree to the Creative Commons Attribution-NonCommercial-ShareAlike (CC BY-NC-SA) license terms, which explain terms governing use, creation of derivative research, and requirements for citing the document.
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
1997
oai:scholarworks.iu.edu:2022/154182014-01-31T20:04:24Zcom_2022_15252com_2022_7911com_2022_7910com_2022_19675com_2022_19673col_2022_15255
Excel worksheet for RIA
Steve Schoech
hormones, endocrinology, testosterone, plasma, assay, RIA lab, calculations, corticosterone, progesterone
A spreadsheet for calculating steroid hormone concentrations from radioimmunoassay results. Calculates concentrations of progesterone, testosterone, estradiol, corticosterone, and dihydrotestosterone.
2013-03-29T15:02:05Z
2013-03-29T15:02:05Z
2013-03-29
Radioimmunoassay Protocol
http://hdl.handle.net/2022/15418
By downloading this document or using any information contained therein, you agree to the Creative Commons Attribution-NonCommercial-ShareAlike (CC BY-NC-SA) license terms, which explain terms governing use, creation of derivative research, and requirements for citing the document.
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
oai:scholarworks.iu.edu:2022/154192021-10-18T09:52:38Zcom_2022_15252com_2022_7911com_2022_7910com_2022_19675com_2022_19673col_2022_15255
Protocol for steroid radioimmunoassay of plasma
Joe Casto, Jodie Jawor, Danielle Whittaker
hormones, endocrinology, corticosterone, cort, assay, lab, plasma, testosterone, progesterone, RIA
This protocol can be used to measure the concentration of steroid hormones in small plasma samples. This protocol has been copied and modified from protocols provided by Dr. John Wingfield at the University of Washington. It has been written for use in the laboratory of Dr. Ellen Ketterson at Indiana University, and therefore the details may require modification if the procedure is performed elsewhere. The general procedure, which takes 4-5 days to complete, is described below. Prepared January 1998 by Joe Casto, modified December 2005 by Jodie Jawor and May 2010 by Danielle Whittaker.
2013-03-29T15:02:06Z
2013-03-29T15:02:06Z
2013-03-29
Radioimmunoassay Protocol
http://hdl.handle.net/2022/15419
By downloading this document or using any information contained therein, you agree to the Creative Commons Attribution-NonCommercial-ShareAlike (CC BY-NC-SA) license terms, which explain terms governing use, creation of derivative research, and requirements for citing the document.
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
1998-2010
oai:scholarworks.iu.edu:2022/154202021-10-18T09:52:39Zcom_2022_15252com_2022_7911com_2022_7910com_2022_19675com_2022_19673col_2022_15255
Steroid Label Clean Up Protocol
Unsure original creator, possibly updated by Kristal Cain?
hormone, endocrinology, assay, lab
Protocol for cleaning radio-labeled steroid hormone
2013-03-29T15:02:06Z
2013-03-29T15:02:06Z
2013-03-29
Radioimmunoassay Protocol
http://hdl.handle.net/2022/15420
By downloading this document or using any information contained therein, you agree to the Creative Commons Attribution-NonCommercial-ShareAlike (CC BY-NC-SA) license terms, which explain terms governing use, creation of derivative research, and requirements for citing the document.
http://creativecommons.org/licenses/by-nc-sa/3.0/us/